Analyzing Graphics: Enzymes - Free Printable

Let’s go step by step to solve this enzyme graphing worksheet.

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Part 1: Label the diagram

We are given a sequence of diagrams showing how an enzyme works with substrates. Let’s label each part based on standard enzyme terminology:

- Enzyme: The big blue shape that doesn’t change overall — it’s the catalyst.
- Substrate(s): The small shapes (green circle, orange triangle) that fit into the enzyme.
- Active site: The specific spot on the enzyme where the substrate binds — looks like a “pocket” or indentation.
- Products: What comes out after the reaction — in this case, the green and orange shapes are now separate (or changed), meaning they’ve been transformed.

Looking at the diagram from left to right:

→ First box (top): These are the substrates before binding.
→ Second box (middle top): Enzyme + substrates bound → this is the enzyme-substrate complex. The active site is where they’re attached.
→ Third box (bottom middle): After reaction, products are released. So these are the products.
→ Bottom left and right: The enzyme itself (blue shape) — same before and after.

So labeling the blanks:

Top row, left blank: Substrates
Top row, right blank: Products
Bottom row, left blank: Enzyme
Bottom row, right blank: Enzyme (again, because enzymes aren’t used up)

Also, the arrow pointing to the pocket on the enzyme = Active site

*(Note: Since we can’t draw here, you’d write those labels directly on your paper next to the correct parts.)*

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Part 2: True or False statements

Let’s evaluate each one:

a) *Enzymes interact with many different substrates.*
→ False. Most enzymes are specific — they only work with one or a few similar substrates. Like a lock and key.

b) *Enzymes change shape after a reaction occurs.*
→ False. Enzymes may temporarily change shape when binding (induced fit), but they return to original shape after releasing products. They don’t stay changed.

c) *An enzyme can be reused with a new substrate.*
→ True. That’s what makes them catalysts — they’re not consumed.

d) *The substrate is changed in the reaction.*
→ True. Substrates become products — chemical bonds break/form.

e) *If the shape of the enzyme changed, it would no longer work.*
→ True. Shape determines function. If denatured (shape ruined), enzyme stops working.

f) *When all substrates are used, the reaction stops.*
→ True. No more reactants = no more product made.

✔ Answers:
a) F
b) F
c) T
d) T
e) T
f) T

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Part 3: Graph analysis — Activation Energy

We have a graph comparing two curves:

- Red curve = without enzyme (higher peak)
- Blue curve = with enzyme (lower peak)

Labels needed:

i) Overall energy released during reaction → This is the difference between start and end energy levels. On the graph, that’s labeled as “D” (vertical drop from reactants to products).

ii) Activation energy with enzyme → That’s the height of the blue hump → labeled “B”

iii) Activation energy without enzyme → Height of red hump → labeled “A”

Wait — let’s double-check the letters on the graph description:

You wrote:

> Identify the letter of the graph that shows:
> i) Overall energy released during reaction → D
> ii) Activation energy with enzyme → B
> iii) Activation energy without enzyme → A

Yes — that matches standard graphs.

In most textbook graphs:

- A = activation energy WITHOUT enzyme (taller barrier)
- B = activation energy WITH enzyme (shorter barrier)
- C = sometimes the difference between A and B (energy saved)
- D = net energy released (exothermic reaction — products lower than reactants)

So yes:

i) D
ii) B
iii) A

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Final Answer:

Part 1 Labels (in order as shown in diagram):
Top left: Substrates
Top right: Products
Bottom left: Enzyme
Bottom right: Enzyme
Arrow to pocket: Active site

Part 2 True/False:
a) False
b) False
c) True
d) True
e) True
f) True

Part 3 Graph Letters:
i) D
ii) B
iii) A