1. The scanning electron microscope produces images by scanning the surface of a specimen with a focused beam of electrons. The electrons interact with the atoms in the specimen, producing various signals that are detected and used to create an image. The primary signal used is the secondary electrons emitted from the surface, which provide information about the topography of the specimen. The image is formed by detecting the intensity of these secondary electrons as the electron beam scans across the surface.
2. The transmission electron microscope produces images by transmitting a beam of electrons through a very thin specimen. The electrons interact with the atoms in the specimen, and the transmitted electrons are then focused by electromagnetic lenses to form an image on a detector. The contrast in the image is due to differences in the density and thickness of the specimen, which affect the amount of electron scattering. The image provides detailed information about the internal structure of the specimen, including the arrangement of atoms and the presence of cellular organelles.
Parent Tip: Review the logic above to help your child master the concept of microscope observation worksheet.