1. Restriction enzyme
2. EcoRI, HindIII
3. Palindromic
4. GATC
5. The DNA fragment would be cut into two pieces: one with the sequence 5’-GATC-3’ and another with 5’-CTAG-3’.
6. Yes, restriction enzymes can be used to cut DNA from other organisms because they recognize specific DNA sequences that are often conserved across species.
7. Palindromes in DNA are sequences that read the same forward and backward on complementary strands. When a restriction enzyme cuts at a palindromic site, it typically makes staggered cuts, producing sticky ends that can base-pair with complementary sticky ends from another DNA fragment cut by the same enzyme, facilitating recombination.
Parent Tip: Review the logic above to help your child master the concept of restriction enzyme worksheet.
