1. The complementary strand is: 5'-ATGCGGCTAGGCCTTA-3'
2. The mRNA sequence transcribed from the given DNA template strand is: 5'-AUGCGGCUAGGCCUUA-3'
3. The amino acid sequence translated from the mRNA is: Met-Arg-Leu-Gly-Leu
4. The stop codon in the mRNA sequence is UAA (not present in the given sequence, so translation would continue until a stop codon is encountered or the end of the mRNA is reached).
5. The type of mutation that changes a codon to a stop codon is a nonsense mutation.
6. The type of mutation that changes one amino acid to another is a missense mutation.
7. The type of mutation that does not change the amino acid sequence is a silent mutation.
8. The type of mutation that inserts or deletes nucleotides in multiples of three is an insertion or deletion mutation (frameshift if not in multiples of three).
9. The type of mutation that changes a single nucleotide is a point mutation.
10. The type of mutation that results in a premature stop codon is a nonsense mutation.
Parent Tip: Review the logic above to help your child master the concept of restriction enzyme worksheet answers.
