1. Number of pieces of DNA: 2
- Cut site: 5' GGATGTTAAC...AATCCTGGGTTAACATCCGCGG 3'
- Fragments: 5' GGATGTTAAC 3' (10 bp) and 5' AATCCTGGGTTAACATCCGCGG 3' (20 bp)
- Type: Blunt cut (3 nucleotides on each side of the dash)
2. Number of pieces of DNA: 2
- Cut site: 5' GGATAATATT...AATTTTAA 3'
- Fragments: 5' GGATAATATT 3' (10 bp) and 5' AATTTTAA 3' (8 bp)
- Type: Blunt cut (3 nucleotides on each side of the dash)
3. Number of pieces of DNA: 2
- Cut site: 5' ACGTGCA/GACGTATTATTAT...GTCATCA 3'
- Fragments: 5' ACGTGCA 3' (7 bp) and 5' GACGTATTATTAT...GTCATCA 3' (23 bp)
- Type: Sticky (cohesive) cut (less than 3 nucleotides on one side of the dash)
4. Number of pieces of DNA: 2
- Cut site: 5' ACGACGTAGTCGACTTATTAT...GTCGACCACCATCA 3'
- Fragments: 5' ACGACGTAGTCGACTTATTAT 3' (19 bp) and 5' GTCGACCACCATCA 3' (14 bp)
- Type: Sticky (cohesive) cut (less than 3 nucleotides on one side of the dash)
5. Number of pieces of DNA: 2
- Cut site: 5' ACG ACGTATTAGAATTCTTAT...CATCA 3'
- Fragments: 5' ACG ACGTATTAGAATTCTTAT 3' (20 bp) and 5' CATCA 3' (5 bp)
- Type: Sticky (cohesive) cut (less than 3 nucleotides on one side of the dash)
Parent Tip: Review the logic above to help your child master the concept of restriction enzymes worksheet.
