1. The DNA extraction buffer contains detergent to break down cell membranes and salt to help separate DNA from proteins.
2. It is important to use strawberries because they are octoploid (have 8 sets of chromosomes), making DNA extraction easier due to higher DNA yield per cell.
3. The role of ethanol is to precipitate DNA out of the aqueous solution, since DNA is insoluble in alcohol.
4. The difference between the filter and the precipitate is that the filter retains solid debris while allowing liquid (containing dissolved DNA) to pass; the precipitate is the visible clump of DNA that forms when ethanol is added.
5. No, there is no human DNA in this experiment. Human DNA would not be present unless a human sample was intentionally added — which is not part of this protocol.
Parent Tip: Review the logic above to help your child master the concept of strawberry dna extraction worksheet.
