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Step-by-step guide to DNA replication with interactive questions.

Diagram illustrating steps of DNA replication with labeled stages and questions about the process.

Diagram illustrating steps of DNA replication with labeled stages and questions about the process.

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Show Answer Key & Explanations Step-by-step solution for: DNA Replication Worksheet | PDF | Dna Replication | Dna
Let’s go step by step to understand what happens during DNA replication.

DNA replication is how a cell makes an exact copy of its DNA before it divides. It happens in three main steps:

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Step 1: Unwinding the DNA

The double helix (twisted ladder shape) of DNA gets unwound and unzipped. An enzyme called helicase breaks the bonds between the base pairs (A-T, G-C), separating the two strands. This creates a “replication fork” — like opening a zipper.

In the first diagram you see, the DNA is still together as one double strand. That’s the starting point.

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Step 2: Building New Strands

Now, each separated strand acts as a template. Another enzyme called DNA polymerase adds new matching bases to each old strand. Remember:
- A always pairs with T
- G always pairs with C

So if one side has “A”, the new side will get “T”. If one side has “G”, the new side gets “C”.

This is shown in the second diagram — you can see the original strands are apart, and new complementary strands are being built alongside them. You might also notice little fragments on one side — that’s because one strand is built continuously, and the other in pieces (called Okazaki fragments). But for now, just know: new strands are being made using the old ones as guides.

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Step 3: Finishing Up

After all the new bases are added, another enzyme (ligase) glues any broken pieces together — especially those little fragments from Step 2. Now you have two complete double helices, each made of one original strand and one brand-new strand. This is called “semi-conservative” replication.

The third diagram shows two full DNA molecules — identical to each other and to the original.

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Final Answer:

1. The DNA molecule is unwound and unzipped into two separate strands. Enzymes break the bonds between base pairs so each strand can be used as a template.

2. New complementary strands are built onto each original strand. DNA polymerase adds matching bases (A with T, G with C) to create two new half-molecules.

3. The new strands are fully connected and sealed. Two identical DNA molecules are formed — each with one original strand and one new strand.
Parent Tip: Review the logic above to help your child master the concept of dna replication activity worksheet.
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