- Original DNA sequence: TAC ACC TTG GCG ACG ACT
- mRNA: AUG UGG AAC CGC UGC UGA
- Amino acids: Met - Trp - Asn - Arg - Cys - Stop
- Mutated DNA sequence #1: TAC ATG TTG GCG ACG ACT
- mRNA: AUG UAC AAC CGC UGC UGA
- Amino acids: Met - Tyr - Asn - Arg - Cys - Stop
- Mutation type: Substitution (point mutation)
- Effect: Missense mutation — changes one amino acid (Trp to Tyr)
- Mutated DNA sequence #2: TAC GAC CTT GGC GAC GAC T
- mRNA: AUG CUG GAA CCG CUG CUG A
- Amino acids: Met - Leu - Glu - Pro - Leu - Leu
- Mutation type: Insertion
- Effect: Frameshift mutation — alters all downstream amino acids and removes stop codon
- Mutated DNA sequence #3: TAC ACC TTG GCG ACG ACT (same as original)
- mRNA: AUG UGG AAC CGC UGC UGA
- Amino acids: Met - Trp - Asn - Arg - Cys - Stop
- Mutation type: None (original sequence)
- Effect: No change
- Mutated DNA sequence #4: TAC ACC TTG GCG ACG ACT (same as original)
- mRNA: AUG UGG AAC CGC UGC UGA
- Amino acids: Met - Trp - Asn - Arg - Cys - Stop
- Mutation type: None (original sequence)
- Effect: No change
Parent Tip: Review the logic above to help your child master the concept of mutations practice worksheet.
