Problem Analysis:
The worksheet focuses on the process of
protein synthesis, which involves several steps: transcription, translation, and the use of codons and anti-codons. The tasks require filling in complementary DNA strands, transcribing DNA to mRNA, translating mRNA codons into amino acids, and understanding the roles of tRNA and codons/anti-codons.
Solution Explanation:
####
Step 1: Fill in the complementary DNA strand using DNA base pairing rules.
-
DNA Base Pairing Rules:
- A pairs with T
- T pairs with A
- C pairs with G
- G pairs with C
Given DNA strand (top):
`ATGGTACGTTAACCCT`
Complementary DNA strand (bottom):
`TACCAATGCATTGGGA`
####
Step 2: Fill in the correct mRNA bases by transcribing the bottom DNA code.
-
Transcription Rules:
- DNA's T is replaced with U in mRNA.
- Other bases remain the same.
Bottom DNA strand:
`TACCAATGCATTGGGA`
mRNA strand:
`UACCUAUUGCUAUGGGU`
####
Step 3: Translate the mRNA codons and find the correct amino acid using the Codon Table.
-
Codon Table: Each codon (a sequence of three nucleotides) corresponds to a specific amino acid.
-
mRNA codons: Break the mRNA strand into groups of three nucleotides.
mRNA strand:
`UAC CUU AUU GCU AUG GGU`
-
Translation:
- `UAC`: Tyrosine (Y)
- `CUU`: Leucine (L)
- `AUU`: Isoleucine (I)
- `GCU`: Alanine (A)
- `AUG`: Methionine (M)
- `GGU`: Glycine (G)
Amino Acid Sequence:
Tyrosine, Leucine, Isoleucine, Alanine, Methionine, Glycine
####
Step 4: Write in the amino acid and the correct anti-codon the tRNA molecule.
-
tRNA Anti-Codon: The anti-codon on tRNA is the reverse complement of the mRNA codon.
-
Anti-Codon Rules:
- A pairs with U
- U pairs with A
- C pairs with G
- G pairs with C
mRNA codons:
`UAC CUU AUU GCU AUG GGU`
tRNA Anti-Codons:
- `UAC` → `AUG`
- `CUU` → `AGA`
- `AUU` → `UAA`
- `GCU` → `CGA`
- `AUG` → `UAC`
- `GGU` → `CCA`
tRNA Anti-Codon Sequence:
`AUG AGA UAA CGA UAC CCA`
####
Step 5: Answer the questions about protein synthesis.
1.
mRNA is synthesized in translation or transcription?
-
Answer: Transcription
2.
mRNA has codons or anti-codons?
-
Answer: Codons
Final Answers:
1.
Complementary DNA strand:
`TACCAATGCATTGGGA`
2.
mRNA strand:
`UACCUAUUGCUAUGGGU`
3.
Amino Acid Sequence:
Tyrosine, Leucine, Isoleucine, Alanine, Methionine, Glycine
4.
tRNA Anti-Codon Sequence:
`AUG AGA UAA CGA UAC CCA`
5.
Questions:
- mRNA is synthesized in
transcription.
- mRNA has
codons.
Final Boxed Answers:
\[
\boxed{
\text{1. TACCAATGCATTGGGA} \\
\text{2. UACCUAUUGCUAUGGGU} \\
\text{3. Tyrosine, Leucine, Isoleucine, Alanine, Methionine, Glycine} \\
\text{4. AUG AGA UAA CGA UAC CCA} \\
\text{5. Transcription, Codons}
}
\]
Parent Tip: Review the logic above to help your child master the concept of protein synthesis activity worksheet.